Microbiology worksheet with answers

Free printable Microbiology worksheet: 8 problems from easy to exam level, with an answer key. Print it or save it as a PDF.

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Name: ____________________   Date: ____________

1.Easy

What colour do Gram-positive bacteria stain in a Gram stain?

  1. AColourless
  2. BGreen
  3. CPurple
  4. DPink
2.Easy

Which is part of the body's first line of defence against infection?

  1. ACytotoxic T cells
  2. BMemory B cells
  3. CIntact skin
  4. DAntibodies
3.Easy

What is sterilisation?

  1. AKilling or removing all microorganisms including spores
  2. BCleaning visible dirt from surfaces
  3. CKilling only bacteria but not viruses
  4. DReducing the number of microorganisms to a level safe for patients
4.Medium

In the lysogenic cycle of a bacteriophage, what happens to the phage DNA?

  1. AIt is immediately used to make new phages that burst the cell
  2. BIt leaves the cell through the pilus
  3. CIt is destroyed by the bacterium
  4. DIt is integrated into the bacterial chromosome as a prophage
5.Medium

Transfer of bacterial genes from one bacterium to another by a virus is called:

  1. ATranscription
  2. BTransduction
  3. CConjugation
  4. DTransformation
6.Medium

Which type of immunity is given by breast milk antibodies?

  1. ANatural passive immunity
  2. BNatural active immunity
  3. CArtificial active immunity
  4. DArtificial passive immunity
7.Hard

Which bacterial toxin causes the muscle spasms of tetanus?

  1. ACholera toxin, which activates adenylate cyclase
  2. BTetanospasmin, which blocks inhibitory neurotransmitter release
  3. CEndotoxin from the outer membrane
  4. DBotulinum toxin, which blocks acetylcholine release at the neuromuscular junction
8.Hard

The minimum inhibitory concentration (MIC) of an antibiotic is:

  1. AThe lowest concentration that prevents visible bacterial growth in vitro
  2. BThe plasma level at which side effects begin
  3. CThe lowest concentration that kills 99.9% of the bacterial inoculum in vitro
  4. DThe highest dose a patient can tolerate

Answer key

  1. 1. C · Purple
  2. 2. C · Intact skin
  3. 3. A · Killing or removing all microorganisms including spores
  4. 4. D · It is integrated into the bacterial chromosome as a prophage
  5. 5. B · Transduction
  6. 6. A · Natural passive immunity
  7. 7. B · Tetanospasmin, which blocks inhibitory neurotransmitter release
  8. 8. A · The lowest concentration that prevents visible bacterial growth in vitro

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